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创新与拿来主义
(2007-11-11 06:59:10)
标签：
健康/保健创新拿来主义 |
分类： 闲情逸致 |
创新与拿来主义看似一对不可调和的矛盾，仔细分析，二者之间存在着必然的联系！这就好像造房子，你不可能不要打地基，直接砌上去就得了！
运用到科研领域，就显得有点说不清了：全部拿来，完全照搬，当然不行！那是在剽窃！一味创新，完全自主，那是在裤裆里面拉二胡！
学习和借鉴是必不可少的环节，拿来的目的是为了创新！解决了二者的位置关系，也就明确了如何拿来，如何创新的问题了！
下面这篇文献，与我们课题组的关系比较密切，其中可能有许多我们目前亟待解决，而又不得要领的问题，仔细研读可能会给我们提供一些有益的思路！
Oxidative stress-induced phosphorylation,
degradation and aggregation of [alpha]-synuclein are linked to upregulated CK2 and cathepsin
D
Takahashi, Makio; Ko, Li-wen; Kulathingal,
Jayanarayan; Jiang, Peizhou; Sevlever, Daniel; Yen, Shu-Hui
C.
European Journal of Neuroscience. 26(4):863-874,
August 2007
Intracellular accumulation of
[alpha]-synuclein ([alpha]-Syn) as filamentous aggregates is a
pathological feature shared by Parkinson's disease, dementia with
Lewy bodies and multiple system atrophy, referred to as
synucleinopathies. To understand the mechanisms underlying
[alpha]-Syn aggregation, we established a tetracycline-off
inducible transfectant (3D5) of neuronal lineage overexpressing
human wild-type [alpha]-Syn. [alpha]-Syn aggregation was initiated
by exposure of 3D5 cells to FeCl2. The exposure led to formation of
[alpha]-Syn inclusions and oligomers of 34, 54, 68 kDa and higher
molecular weights. The oligomers displayed immunoreactivity with
antibodies to the amino-, but not to the carboxyl(C)-, terminus of
[alpha]-Syn, indicating that C-terminally truncated [alpha]-Syn is
a major component of oligomers. FeCl2 exposure also promoted
accumulation of S129 phosphorylated monomeric [alpha]-Syn
(P[alpha]-Syn) and casein kinase 2 (CK2); however,
G-protein-coupled receptor kinase 2 was reduced. Treatment of
FeCl2-exposed cells with CK2 inhibitors (DRB or TBB) led to
decreased formation of [alpha]-Syn inclusions, oligomers and
P[alpha]-Syn. FeCl2 exposure also enhanced the activity/level of
cathepsin D. Treatment of the FeCl2-exposed cells with pepstatin A
or NH4Cl led to reduced formation of oligomers/inclusions as well
as of ~ 10 and 12 kDa truncated [alpha]-Syn. Our results indicate
that [alpha]-Syn phosphorylation caused by FeCl2 is due to CK2
upregulation, and that lysosomal proteases may have a role in
producing truncated [alpha]-Syn for oligomer
assembly.
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