猪细小病毒的临床症状及防控与治疗！

猪细小病毒是Parvovirus属的微生物。中监所，PPV7909。原始分离：北京，猪肾。制备材料：感染PK15细胞培养物。培养：37，3天。效应：致细胞病变。冻干保存于—70。宿主范围：猪和PK15、IBRS。主要用途为研究，具体用途为制备抗血清。

菌株简介

平台编号：Bio-49930

规格：Frozen

拉丁属名：Porcine Parvovirus NADL-2

菌株名称：猪细小病毒

用途：ATCC原装进口

注意事项：仅用于科学研究或者工业应用等非医疗目的不可用于人类或动物的临床诊断或治疗，非药用，非食用（产品信息以出库为准）

详细描述

Porcine parvovirus NADL-2 拉丁名

ATCC VR-742 编号

Classification Parvoviridae, Parvovirus, Porcine parvovirus

Deposited As Porcine parvovirus

Agent Porcine parvovirus NADL-2

Strain NADL-2

Biosafety Level 安全等级 2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format frozen 1 mL per vial

Storage Conditions -70°C or colder

Comments

At the time this deposit was made, TC of other species had not been extensively evaluated as viral hosts.

HA activity in chicken, rat, cat, and GP RBC at 22°C and 4°C (HA activity is four fold higher at 4°C than at 22°C for cat RBC). No HA activity in pig, hamster, sheep or cattle RBC. Virus must be freeze-thawed 3 times before inoculating and/or passing with fresh cells. CPE most pronounced when cells are exposed to the virus just before or after subculturing.

Effect on Host

CPE, nuclear inclusions and degeneration

Recommended Host

ST (ATCC CRL-1746)

Growth Conditions Temperature 37°C

Recommendations for Infection: This product is produced by co-cultivation of virus with fresh host cells. Prepare a bulk cell suspension the day of inoculation. Seed culture vessels at 30-50% confluency. Calculate the volume of virus needed to achieve an optimal MOI (e.g. 1:100 dilution) and then dilute virus in virus growth medium to prepare the virus inoculum. Add virus inoculum to culture vessels. Incubate for 24 hours at 37°C in a humidified 5% CO2 atmosphere. Aspirate virus growth medium to remove any traces of DMSO and then add fresh virus growth medium to cultures. Continue incubation.

Incubation: 3 -5 days

Effect on Host

CPE, nuclear inclusions and degeneration

Name of Depositor WL Mengeling

Source Porcine leukocytes from blood samples collected at an abattoir

References

Mengeling WL. Porcine parvovirus: properties and prevalence of a strain isolated in the United States. Am. J. Vet. Res. 33: 2239-2248, 1972. PubMed: 4628211

猪细小病毒的症状和治疗防控

猪细小病毒是一种传染病，会引发母猪繁殖机能的障碍，这个猪病在后期其实没有什么特别明显的症状，就是因为这一点，让很多篇养猪户朋友都吃过大亏。下面一起学习了解下猪细小病毒的症状以及防控治疗。

一、猪细小病毒病临床症状

猪细小病毒病可以导致妊娠母猪繁殖异常或者失去繁殖能力。患病母猪常发生不孕、流产，产死胎、木乃伊胎、畸形胎或者弱胎。部分妊娠母猪在染病初期会有体温升高，也有可能造成屡配不孕、发情异常等。未妊娠母猪和公猪感染此病，通常没有明显的临床症状，公猪的精液带毒，不影响受精率。

二、猪细小病毒病的防控和治疗

1、免疫接种灭

活疫苗有猪细小病毒油乳剂灭活疫苗和猪细小病毒氢氧化铝灭活疫苗。猪细小病毒油乳剂灭活疫苗肌肉注射，种公猪和母猪要在6月龄和配种前半个月进行接种。成年公猪和经产母猪每年要接种1次，但是此疫苗不适合妊娠母猪。氢氧化铝灭活疫苗可在母猪妊娠前几周接种，可以保证妊娠期都有抗体。初产母猪在6月龄时母源抗体会消失，应在这时接种疫苗。如果不打算马上配种，可以先不免疫，在7月龄后再进行免疫。对于发病的猪场，每年要进行2次抗体检测，对抗体浓度低的猪要及时补接疫苗。

2、加强饲养管理

猪场按时清扫卫生，定期对猪舍和饲喂用具消毒。猪场应实行封闭式管理，对来往的人员和车辆进行消毒。猪场应坚持自繁自养模式，如果必须引种的话，一定要从非疫区引进，而且种猪需要有检疫证明。新引进来的种猪需要先隔离一段时间，观察无问题后进行合群。综上所述，猪细小病毒病传播率高、发病广泛，其对养猪业造成的影响巨大，严重影响养猪业效益。此病暂无特效药，所以免疫接种是最有效的防控手段。养殖人员应提高防病意识，积极采取措施预防此病，制定合理的消毒计划和免疫计划，降低此病的发病率。