还在为转染实验发愁吗？

细胞太脆弱，经不住折腾，

不能含血清，苦命地反复换液，

转染效率低，实验各种不理想。

面对一次次的挫败，

什么时候我才能翻身做主人，

漂漂亮亮转细胞。

细胞转染试剂

全式金转染试剂EL、PL，凭借自身高效的产品性能，超强的细胞广适性，助你轻松应对转染实验！

TransIntro^® EL Transfection Reagent

该产品是一种非脂质体转染试剂，适用于将DNA和RNA转入真核细胞。该试剂转染效率高，细胞毒性低，转染时可以有血清和抗生素的存在，对贴壁细胞和悬浮细胞均可转染。

  1、产品特点

 转染效率高：提升对多种难转染细胞的转染效率。

 细胞毒性低：对细胞生理过程影响小。

 操作简单：转染后无需更换培养基，可带血清和抗生素转染。

 适用范围广：DNA和RNA均可转染。

2、实验数据

3、已成功转染细胞

TransIntro^® PL Transfection Reagent

该产品是一种脂质体转染试剂，适用于将DNA转入真核细胞，针对应用广泛的难转染细胞，有效提高DNA的转染效率。

   1、产品特点

 对于肺癌、结直肠癌、肝癌、胰腺癌等多种肿瘤细胞系的转染高效稳定，有助于建立稳定可靠的细胞模型。

 细胞毒性低，对细胞生理过程影响小。

 抗生素对转染效率没有影响。

2、实验数据

3、已成功转染细胞

产品信息

参与研究领域

全式金转染试剂自上市以来，备受广大科研工作者的青睐，目前已被广泛应用于多个研究领域。

1、 胆固醇衍生物诱导细胞自噬的机制研究

2、先天免疫反应研究

本研究揭示了RUVBL1/2作为巨噬细胞促炎反应的重要组成部分，参与表观遗传调控的分子机制，以及在促炎通路被异常激活引发疾病的过程中，RUVBL1/2抑制剂表现出一定的治疗潜能，为先天免疫的研究提供了新思路，其中转染细胞为RAW 264.7巨噬细胞。

3、癌细胞调控机制研究

本研究揭示了CIB-6通过靶向STAT3的SH2结构域，可抑制STAT3的磷酸化和核易位，进而调控STAT3介导的β-TrCP/β-catenin/NF-κB轴过程，在体内外有效抑制HCC肿瘤的发生，为HCC的治疗提供新策略，其中转染细胞为HepG2细胞。

4、抗心肌肥大的靶点研究

本研究中，通过调控miR-214的表达，抑制SIRT3参与血管紧张素II诱导的心肌肥大，进而导致线粒体功能障碍，揭示了miR-214可能为抗心肌肥大治疗的一个潜在的干预靶点，其中转染细胞为心肌细胞AC16和NRCMs。

5、抗病毒治疗的新细胞靶点研究

本研究揭示了mTOR信号通路通过PGC-1α依赖的SIRT3调控ISKNV感染细胞的还原性谷氨酰胺代谢，为ISKNV的发病机制和抗病毒治疗策略提供了新的见解，其中转染细胞为CPB细胞。

助力发表的部分文章

• Fu X, Li K, Niu Y, et al. The mTOR/PGC-1α/SIRT3 Pathway Drives Reductive Glutamine Metabolism to Reduce Oxidative Stress Caused by ISKNV in CPB Cells[J]. Microbiology Spectrum, 2022, 10(1): e02310-21.

• Wu W, Luo M, Li K, et al. Cholesterol derivatives induce dephosphorylation of the histone deacetylases Rpd3/HDAC1 to upregulate autophagy[J]. Autophagy, 2021, 17(2): 512-528.

• Zhang R, Cheung C Y, Seo S U, et al. RUVBL1/2 Complex Regulates Pro-Inflammatory Responses in Macrophages via Regulating Histone H3K4 Trimethylation[J]. Frontiers in immunology, 2021, 12: 2074.

• Liu Y, Cheng A, Wang M, et al. Duck Hepatitis A Virus Type 1 Induces eIF2α Phosphorylation-Dependent Cellular Translation Shutoff via PERK/GCN2[J]. Frontiers in microbiology, 2021, 12: 746.

• Ding Y, Zhang Y, Lu J, et al. MicroRNA-214 contributes to Ang II-induced cardiac hypertrophy by targeting SIRT3 to provoke mitochondrial malfunction[J]. Acta Pharmacologica Sinica, 2021, 42(9): 1422-1436.

• Jiang J, Liu T, He X, et al. Silencing of KIF18B restricts proliferation and invasion and enhances the chemosensitivity of breast cancer via modulating Akt/GSK3β/βcatenin pathway[J]. BioFactors, 2021, 47(5): 754-767.

• Peng T, Wonganan O, Zhang Z, et al. A 2-Benzylmalonate Derivative as STAT3 Inhibitor Suppresses Tumor Growth in Hepatocellular Carcinoma by Upregulating β-TrCP E3 Ubiquitin Ligase[J]. International journal of molecular sciences, 2021, 22(7): 3354.

• Chen Y, Zhu S, Hu J, et al. gga-miR-1603 and gga-miR-1794 directly target viral L gene and function as a broad-spectrum antiviral factor against NDV replication[J]. Virulence, 2021, 12(1): 45-56.

• Hu J, Wang J, Li C, et al. Fructose1, 6bisphosphatase aggravates oxidative stressinduced apoptosis in asthma by suppressing the Nrf2 pathway[J]. Journal of cellular and molecular medicine, 2021, 25(11): 5001-5014.

• Cui Q, Yan L. Tripartite motif-containing protein 16 protects against myocardial ischemia/reperfusion injury by affecting the Keap1/Nrf2 axis[J]. Cell and tissue research, 2021, 386(2): 349-363.

• Wu W, Li K, Guo S, et al. P300/HDAC1 regulates the acetylation/deacetylation and autophagic activities of LC3/Atg8–PE ubiquitin-like system[J]. Cell death discovery, 2021, 7(1): 1-15.

• Hu S, Li Y, Wang B, et al. TRIM38 protects chondrocytes from IL-1β-induced apoptosis and degeneration via negatively modulating nuclear factor (NF)-κB signaling[J]. International Immunopharmacology, 2021, 99: 108048.

• Zhu F, Shao J, Tian Y, et al. Sulfiredoxin-1 protects retinal ganglion cells from high glucose-induced oxidative stress and inflammatory injury by potentiating Nrf2 signaling via the Akt/GSK-3β pathway[J]. International Immunopharmacology, 2021, 101: 108221.

• Zhang L, Zhu T, He F, et al. Senescence marker protein 30 (SMP30) protects against high glucose-induced apoptosis, oxidative stress and inflammatory response in retinal ganglion cells by enhancing Nrf2 activation via regulation of Akt/GSK-3β pathway[J]. International Immunopharmacology, 2021, 101: 108238.

• Xiang C, Huang M, Xiong T, et al. Transcriptomic analysis and functional characterization reveal the duck interferon regulatory factor 1 as an important restriction factor in the replication of Tembusu virus[J]. Frontiers in microbiology, 2020: 2069.

• Sun Y, He L, Wang T, et al. Activation of p62-Keap1-Nrf2 pathway protects 6-hydroxydopamine-induced ferroptosis in dopaminergic cells[J]. Molecular Neurobiology, 2020, 57(11): 4628-4641.

• Wang T, Merits A, Wu Y, et al. Cis-acting sequences and secondary structures in untranslated regions of duck tembusu virus RNA are important for cap-independent translation and viral proliferation[J]. Journal of Virology, 2020, 94(16): e00906-20.

• Yuan L X, Liang J Q, Zhu Q C, et al. A gammacoronavirus, avian infectious bronchitis virus, and an alphacoronavirus, porcine epidemic diarrhea virus, exploit a cell survival strategy by upregulating cFOS to promote virus replication[J]. Journal of Virology, 2020, 95(4): e02107-20.

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