加载中…SDS-PAGE胶配方
(2011-08-15 09:54:09)| 分类： 小常识 |
SDS-PAGE浓缩胶配方（5% Acrylamide）
|
各组分名称 |
各种凝胶体积所对应的各种组分的取样量 |
|||||||
|
1mL |
2 mL |
3 mL |
4 mL |
5 mL |
6 mL |
8 mL |
10 mL |
|
|
H2O |
0.68 |
1.4 |
2.1 |
2.7 |
3.4 |
4.1 |
5.5 |
6.8 |
|
30% Acrylamide |
0.17 |
0.33 |
0.5 |
0.67 |
0.83 |
1.0 |
1.3 |
1.7 |
|
1.0 M Tris-HCl(Ph6.8) |
0.13 |
0.25 |
0.38 |
0.5 |
0.63 |
0.75 |
1.0 |
1.25 |
|
10% SDS |
0.01 |
0.02 |
0.03 |
0.04 |
0.05 |
0.06 |
0.08 |
0.1 |
|
10% Ammonium persulfate |
0.01 |
0.02 |
0.03 |
0.04 |
0.05 |
0.06 |
0.08 |
0.1 |
|
TEMED |
0.001 |
0.002 |
0.003 |
0.004 |
0.005 |
0.006 |
0.008 |
0.01 |
SDS-PAGE分离胶配方
|
各组分名称 |
各种凝胶体积所对应的各种组分的取样量 |
|||||||
|
6% Gel |
5mL |
10 mL |
15 mL |
20 mL |
25 mL |
30 mL |
40 mL |
50 mL |
|
H2O |
2.6 |
5.3 |
7.9 |
10.6 |
31.2 |
15.9 |
21.2 |
26.5 |
|
30% Acrylamide |
1.0 |
2.0 |
3.0 |
4.0 |
5.0 |
6.0 |
8.0 |
10.0 |
|
1.5 M Tris-HCl(pH8.8) |
1.3 |
2.5 |
3.8 |
5.0 |
6.3 |
7.5 |
10.0 |
12.5 |
|
10% SDS |
0.05 |
0.1 |
0.15 |
0.2 |
0.25 |
0.3 |
0.4 |
0.5 |
|
10% Ammonium persulfate |
0.05 |
0.1 |
0.15 |
0.2 |
0.25 |
0.3 |
0.4 |
0.5 |
|
TEMED |
0.004 |
0.008 |
0.012 |
0.016 |
0.02 |
0.024 |
0.032 |
0.04 |
|
8% Gel |
|
|
|
|
|
|
|
|
|
H2O |
2.3 |
4.6 |
6.9 |
9.3 |
11.5 |
13.9 |
18.5 |
23.2 |
|
30% Acrylamide |
1.3 |
2.7 |
4.0 |
5.3 |
6.7 |
8.0 |
10.7 |
13.3 |
|
1.5 M Tris-HCl(pH8.8) |
1.3 |
2.5 |
3.8 |
5.0 |
6.3 |
7.5 |
10.0 |
12.5 |
|
10% SDS |
0.05 |
0.1 |
0.15 |
0.2 |
0.25 |
0.3 |
0.4 |
0.5 |
|
10% Ammonium persulfate |
0.05 |
0.1 |
0.15 |
0.2 |
0.25 |
0.3 |
0.4 |
0.5 |
|
TEMED |
0.003 |
0.006 |
0.009 |
0.012 |
0.015 |
0.018 |
0.024 |
0.03 |
|
10% Gel |
|
|
|
|
|
|
|
|
|
H2O |
1.9 |
4.0 |
5.9 |
7.9 |
9.9 |
11.9 |
15.9 |
19.8 |
|
30% Acrylamide |
1.7 |
3.3 |
5.0 |
6.7 |
8.3 |
10.0 |
13.3 |
16.7 |
|
1.5 M Tris-HCl(pH8.8) |
1.3 |
2.5 |
3.8 |
5.0 |
6.3 |
7.5 |
10.0 |
12.5 |
|
10% SDS |
0.005 |
0.1 |
0.15 |
0.2 |
0.25 |
0.3 |
0.4 |
0.5 |
|
10% Ammonium persulfate |
0.005 |
0.1 |
0.15 |
0.2 |
0.25 |
0.3 |
0.4 |
0.5 |
|
TEMED |
0.002 |
0.004 |
0.006 |
0.008 |
0.01 |
0.012 |
0.016 |
0.02 |
|
12% Gel |
|
|
|
|
|
|
|
|
|
H2O |
1.6 |
3.3 |
4.9 |
6.6 |
8.2 |
9.9 |
13.2 |
16.5 |
|
30% Acrylamide |
2.0 |
4.0 |
6.0 |
8.0 |
10.0 |
12.0 |
16.0 |
20.0 |
|
1.5 M Tris-HCl(pH8.8) |
1.3 |
2.5 |
3.8 |
5.0 |
6.3 |
7.5 |
10.0 |
12.5 |
|
10% SDS |
0.005 |
0.1 |
0.15 |
0.2 |
0.25 |
0.3 |
0.4 |
0.5 |
|
10% Ammonium persulfate |
0.005 |
0.1 |
0.15 |
0.2 |
0.25 |
0.3 |
0.4 |
0.5 |
|
TEMED |
0.002 |
0.004 |
0.006 |
0.008 |
0.01 |
0.012 |
0.016 |
0.02 |
|
15% Gel |
|
|
|
|
|
|
|
|
|
H2O |
1.1 |
2.3 |
3.4 |
4.6 |
5.7 |
6.9 |
9.2 |
11.5 |
|
30% Acrylamide |
2.5 |
5.0 |
7.5 |
10.0 |
12.5 |
15.0 |
20.0 |
25.0 |
|
1.5 M Tris-HCl(pH8.8) |
1.3 |
2.5 |
3.8 |
5.0 |
6.3 |
7.5 |
10.0 |
12.5 |
|
10% SDS |
0.005 |
0.1 |
0.15 |
0.2 |
0.25 |
0.3 |
0.4 |
0.5 |
|
10% Ammonium persulfate |
0.005 |
0.1 |
0.15 |
0.2 |
0.25 |
0.3 |
0.4 |
0.5 |
|
TEMED |
0.002 |
0.004 |
0.006 |
0.008 |
0.01 |
0.012 |
0.016 |
0.02 |
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