加载中…Ion Torrent测序平台介绍
(2012-08-09 14:36:08)
标签：
iontorrent测序第三代测序平台介绍教育 |
分类： 生物及医药信息 |
简单Simple natural chemistry helps result in unmatched uniformity of coverage. Ion Torrent™ uses simple sequencing chemistry including natural nucleotides, no enzymatic cascade, no fluorescence, no chemiluminescence, no optics, no light: The Chip is the Machine.™ Ion Torrent™ pairs proprietary semiconductor technology with a simple sequencing chemistry that is based on a well-characterized biochemical process. |
步骤 1
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In nature, when a nucleotide is incorporated into a strand of DNA by a polymerase, a hydrogen ion is released as a byproduct. |
步骤 2
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Ion Torrent™ uses a high-density array of micro-machined wells to perform this biochemical process in a massively parallel way. Each well holds a different DNA template. Beneath the wells is an ion-sensitive layer and beneath that a proprietary Ion sensor. |
步骤 3
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Here’s how the technology is used to call a base: If a nucleotide, for example a C, is added to a DNA template and is then incorporated into a strand of DNA, a hydrogen ion will be released. The charge from that ion will change the pH of the solution, which can be detected by our proprietary ion sensor. Our sequencer - essentially the world's smallest solid-state pH meter - will call the base, going directly from chemical information to digital information. |
Step Four
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The Ion Personal Genome Machine™ (PGM™) sequencer then sequentially floods the chip with one nucleotide after another. If the next nucleotide that floods the chip is not a match, no voltage change will be recorded and no base will be called. |
Step Five
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The semiconductor will inevitably transform the life sciences, just as it has transformed every other industry it has touched. By creating a direct connection between chemical and digital information, Ion Torrent™ will democratize research, providing a fast, simple, scalable sequencing solution that every lab can afford. Eventually, Ion Torrent Technology™ will also be able to provide diagnostics that are less expensive and more reliable, improving human health around the world.
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