安全无毒的EB替代品，GoodViewTM 核酸染料_skypple

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安全无毒的EB替代品，GoodViewTM 核酸染料

(2013-05-06 23:43:12)

标签：

eb

核酸燃料

文化

分类： Amresco

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1. EZ-Vision DNA Dye as Loading Buffer
EZ-Vision DNA染液 + 6x上样缓冲液（混合在一个管里）

无需配胶时加染料
只需上样、跑胶，电泳后即可在紫外下观察DNA条带
用量：6X , 即1ul EZ-Vision + 5ul DNA样品
提供三种上样缓冲液，分别含1、2、3条电泳指示带

品名及描述	级别	货号	包装	价格（元）
EZ Vision One DNA Dye as Loading Buffer EZ Vision DNA染料上样缓冲液 (含1条指示带) 指示带近似位置为：10bp	生物技术级生物技术级生物技术级	amresco N472-Q amresco N472 amresco N472	0.5ml 1ml 5x 1ml	408 450 1806
EZ Vision Two DNA Dye as Loading Buffer EZ Vision DNA染料上样缓冲液 (含2条指示带) 指示带近似位置为：400bp， 4000	生物技术级	amresco N650	5x 1ml	1625
EZ Vision Three DNA Dye as Loading Buffer EZ Vision DNA染料上样缓冲液 (含3条指示带) 指示带近似位置为：10bp, 400bp, 4000bp	生物技术级生物技术级	amresco N313 amresco N313	1ml 5x 1ml	385 1701
EZ Vision Sample Kit EZ Vision DNA套装含N472-1ml, N313-1ml	生物技术级	amresco N473-2管	1套	755
EZ Vision Sample Kit EZ Vision DNA套装含N472-1ml, N650-1ml, N473-1ml	生物技术级	amresco N473-3管	1套	1026

2. EZ-Vision In-Gel Solution, 10,000 x
EZ-Vision 染液, 10,000 x （加在胶内，与EB用法一样）

与EB 用法一样，溶胶时加在胶内，稀释比例为1：10000。

品名及描述	级别	货号	包装	价格（元）
EZ Vision In-Gel Solution, 10000 x EZ Vision 染液， 10000 x	生物技术级	amresco N391-0.5ml	0.5ml	1132

EZ-Vision, DNA Dye as Loading Buffer, 6X
EZ-Vision is a non-mutagenic fluorescent reagent that produces instant visualization of DNA bands upon UV illumination of agarose gels. EZ-Vision is non-mutagenic and non-toxic with no hazardous shipping, handling or disposal costs. Supplied in AMRESCO’s 6X DNA Loading Buffer, EZ-Vision forms a tight complex with the sample DNA and co-migrates with it during electrophoresis. Post-run staining and destaining is completely eliminated and results can be visualized immediately after the run by placing the gel on a standard UV transilluminator. It is ideal for applications needing rapid DNA band visualization and for environments requiring a safe, non-hazardous alternative to Ethidium Bromide. EZ-Vision is available in 3 versions that differ only by the tracking dyes included in the loading buffer. EZ-Vision Three contains 3 tracking dyes that migrate at 4,000 bp, 400 bp, and 10 bp. EZ-Vision Two contains 2 tracking dyes that migrate at 4,000 bp and 400 bp. EZ-Vision One contains only a single fast-running tracking dye that migrates at approximately 10 bp in a 1% agarose gel.

Available with 3 different tracking dye options!

http://www.amresco-inc.com/media.acux/264c07cd-689f-4fd0-acd8-205b7b1a9a31-x250核酸染料" />

EZ-Vision One, Two and Three. Left image: 1% TAE agarose gel showing the fluorescence of AMRESCO’s 1kb Ladder (K180-250μl) stained with EZVision One (lane 1), EZ-Vision Two (lane 2) and EZ-Vision Three (lane3) captured with Syngene GBox-HR Gel Doc System using SP filter selection. Right image: Digital camera photograph of the same gel as left image, showing the colors and migration position of the one fast migrating tracking dye of EZ-Vision One (lane 1), the two tracking dyes of EZ-Vision Two (lane2), and the three tracking dyes of EZ-Vision Three (lane 3).

EZ-Vision sensitivity compared to Ethidium Bromide

http://www.amresco-inc.com/media.acux/3799f6e2-079d-430b-a355-f05fea2575a5-450x核酸染料" />

DNA MW Marker, 1KB Ladder was mixed with EZ-Vision Two, DNA Dye as Loading Buffer, 6X or a loading buffer containing 0.5 μg/mL ethidium bromide. After electrophoresis, the gel was visualized on a standard UV illuminator.

EZ-Vision Environmental Toxicity Testing

http://www.amresco-inc.com/media.acux/c39e4962-f3cf-4c4d-b774-03a5a1641c45-250x核酸染料" />

EZ-Vision environmental hazard testing was determined by the CCR Title 22 Fathead Minnow Hazardous Waste Screen Bioassay. Both EZ-Vision Two and EZ-Vision? Three were determined non-hazardous with LC50 > 750 mg/l.

Relative migration of prestained samples of dsDNA in 1% agarose gel

http://www.amresco-inc.com/media.acux/385a3255-feb2-497e-9714-3d5cc9a6b60e-x250核酸染料" />

The data shows that EZ-Vision has much less effect on DNA migration than Ethidium Bromide or Competitor 2 Green Dye. Samples stained with EZ-Vision are not impeded and migrate similar to dsDNA that is stained post electrophoresis.

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