荧光标记基团的激发和发射波长是广大科研工作者最关心的内容.        

下面就我们大家常用的多肽标记的各种荧光基团数据参数提供给大家. 

1-PBA (Ex: 341nm, Em: 380nm)       MCA  (Ex: 353nm, Em: 442nm)      EDANS (Ex: 335nm, Em: 493nm) 

                                           
NBD (Ex: 464nm, Em: 512nm)         FITC(Ex: 494nm, Em: 518nm)     5-FAM (Ex: 494nm, Em: 522nm)

Dansyl (Ex: 336nm, Em: 534nm)    Rhodamine B (Ex: 555nm, Em: 580nm)  TAMRA (Ex: 560nm, Em: 582nm)

从发射波长相近的角度考虑,上述荧光基团在用Alex Fluor替换时,可参照下表

Alexa Fluor dye                      Alternatives common dyes

Alexa Fluor 350                           Coumarin (MCA, AMC)

Alexa Fluor 488                           Cy2 or Fluorescein (FITC, FAM)

Alexa Fluor 555                           Cy3 or Tetramethylrhodamine (TRMRA)

Alexa Fluor 568                           Rhodamine Red

Alexa Fluor 594                           Texas Red

Alexa Fluor 647                           Cy5

Alexa Fluor 680                           Cy5.5

Alexa Fluor 750                           Cy7