ATCC 43015 鸟分枝杆菌副结核亚种知识解析！

菌株简介

平台编号：Bio-81890

规格：Freeze-Dried

拉丁属名：Mycobacterium Avium Subsp. Paratuberculosis

菌株名称：鸟分枝杆菌副结核亚种；副结核分枝杆菌

用途：ATCC原装进口

注意事项：仅用于科学研究或者工业应用等非医疗目的不可用于人类或动物的临床诊断或治疗，非药用，非食用（产品信息以出库为准）

详细描述

Mycobacterium avium subsp. paratuberculosis (Bergey et al.) Thorel et al. 拉丁名

(ATCC® 43015™) 统一编号

Deposited As Mycobacterium paratuberculosis Bergey et al.

Strain Designations 菌株别名 Linda [CIP 103965]

Application 用途 Characterization Isolation Ileum of 15-year-old girl with Crohn's disease, Rhode Island.

Biosafety Level 生物安全等级 2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format 提供形式 freeze-dried

Preceptrol® no

Type Strain 模式菌株 no

Comments 注释 Intestinal multiplication in gnotobiotic mice

Medium 培养基 ATCC® Medium 1507: Supplemented Middlebrook

Growth Conditions 生长条件

Temperature 培养温度 : 37.0°C

Name of Depositor 寄存人 RJ Chiodini

Isolation Ileum of 15-year-old girl with Crohn's disease, Rhode Island.

References 参考文献 Hamilton HL, et al. Intestinal multiplication of Mycobacterium paratuberculosis in athymic nude gnotobiotic mice. Infect. Immun. 57: 225-230, 1989. PubMed: 2909488

Chiodini RJ, et al. Characteristics of an unclassified Mycobacterium species isolated from patients with Crohn's disease. J. Clin. Microbiol. 20: 966-971, 1984. PubMed: 6511878

McFadden JJ, et al. Crohn's disease-isolated mycobacteria are identical to Mycobacterium paratuberculosis, as determined by DNA probes that distinguish between mycobacterial species. J. Clin. Microbiol. 25: 796-801, 1987. PubMed: 2884232

Chiodini RJ, et al. In vitro antimicrobial susceptibility of a Mycobacterium sp. isolated from patients with Crohn's disease. Antimicrob. Agents Chemother. 26: 930-932, 1984. PubMed: 6524906

Chiodini RJ, et al. Possible role of mycobacteria in inflammatory bowel disease. I. An unclassified Mycobacterium species isolated from patients with Crohn's disease. Dig. Dis. Sci. 29: 1073-1079, 1984. PubMed: 6499624

培养及打管说明

1、安瓿瓶开封：用浸过75％酒精的脱脂棉擦净安瓿管，用火焰加热其顶端，滴少量无菌水至加热顶端使之破裂，用锉刀或者镊子敲下已破裂的安瓿管顶端。

2、菌株恢复培养：用无菌吸管吸取0.3--0.5ml适宜的液体培养基，滴入安瓿管内，轻轻振荡，使冻干菌体溶解呈悬浮状。吸取全部菌体悬浮液，移植于1-2支建议的培养基试管中，并在建议的条件下培养。

3、注意事项：菌种活化前，请将安瓿管保存在6-10的环境下，某些菌种经过冷冻干燥保存后，延迟期较长，需要连续两次继代培养才能正常生长。

4、复苏后的菌种在传1-2代后使用。

5、暂不启开的安瓿及复苏后需保藏的斜面应于4中保藏。