ATCC 30556 寇氏隐甲藻 百欧博伟生物

菌种简介

平台编号：bio-67695

规格：冷冻的

拉丁属名：Crypthecodinium cohnii (Seligo) Javornicky

菌株名称：寇氏隐甲藻

注意事项：仅用于科学研究或者工业应用等非医疗目的，不可用于人类或动物的临床诊断或治疗，非药用，非食用

产品概述

Crypthecodinium cohnii (Seligo) Javornicky 菌株拉丁名

(ATCC? 30556?) 菌株编号

Strain Designations 菌株别名 : To (Tolo) /

Depositor: CA Beam, M Himes /

Biosafety Level 生物安全等级 : 1

Strain Designations 菌株别名 To (Tolo)

Application 用途 Biofuel production

Biosafety Level 生物安全等级 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Isolation 分离源 Shoreline organic debris, Tolo Harbor, Hong Kong, 1976

Product Format 提供形式 frozen

Storage Conditions 保藏方法

Frozen Cultures 冷冻培养 : -70 for 1 week; liquid N2 vapor for long term

storage 保藏规格 Freeze-dried

Cultures: 2-8

Live Cultures 活菌 : See Protocols section for handling information

Type Strain 模式菌株 no

Comments 注释 Minor sibling species Genetic diversification Sexuality and meiosis

Medium 培养基 ATCC? Medium 460: A2E6 medium

Growth Conditions 生长条件

Temperature 培养温度 : 20 to 25

Culture System: Axenic Cryopreservation Harvest and Preservation

1.Harvest cells from cultures which are at or near peak density.Aseptically transfer cells to 15 mL plastic centrifuge tubes and centrifuge at ~150 x g for 5 min.

2.Adjust the concentration of cells to 2 x 106/mL with fresh medium, then dilute to half this concentration by adding an equal amount of a 15% (v/v) sterile glycerol solution in fresh medium.

3.Dispense in 0.5 mL aliquots into 1.0 mL to 2.0 mL sterile plastic screw-capped cryules (special plastic vials for cryopreservation). The time from mixing of the cell suspension and the glycerol solution, before the cooling cycle begins, should be no less than 15 min and no greater than 30 min.

4.Place vials in a controlled-rate freezing unit. From room temperature, cool at -1/min to -40. If freezing unit can compensate for the heat of fusion, maintain rate at -1 C/min through heat of fusion. At -40 plunge ampules into liquid nitrogen.

Alternatively, place the vials in a Nalgene 1 freezing apparatus. Place the apparatus at -80 for 1.5 to 2 hours and then plunge ampules into liquid nitrogen. (The cooling rate in this apparatus is approximately -1/min.)

5.The frozen preparations should be stored in either the vapor or liquid phase of a nitrogen refrigerator. Frozen preparations stored below -130 are stabile indefinitely. Those stored at temperatures above -130 are progressively less stabile as the storage temperature is elevated. Vials can be stored between -80 and -70 for no longer than one week.

6.To establish a culture from the frozen state place an ampule in a water bath set at 35. Immerse the vial to a level just above the surface of the frozen material. Do not agitate the vial.

7.Immediately after thawing, do not leave in the water bath; aseptically remove the contents of the ampule and transfer to a fresh tube (or T25 flask) containing 5 mL of ATCC Medium 460. Incubate culture (if in a tube, incubate vertically) at 20 to 25 with the cap loosened one-half turn. Subculture every 10 to 14 days.

Name of Depositor 寄存人 CA Beam, M Himes

Year of Origin 1976

打管说明

1、安瓿瓶开封：用浸过75％酒精的脱脂棉擦净安瓿管，用火焰加热其顶端，滴少量无菌水至加热顶端使之破裂，用锉刀或者镊子敲下已破裂的安瓿管顶端。

2、菌株恢复培养：用无菌吸管吸取0.3--0.5ml适宜的液体培养基，滴入安瓿管内，轻轻振荡，使冻干菌体溶解呈悬浮状。吸取全部菌体悬浮液，移植于1-2支建议的培养基试管中，并在建议的条件下培养。

3、注意事项：菌种活化前，请将安瓿管保存在6-10的环境下，某些菌种经过冷冻干燥保存后，延迟期较长，需要连续两次继代培养才能正常生长。

4、复苏后的菌种在传1-2代后使用。

5、暂不启开的安瓿及复苏后需保藏的斜面应于4中保藏。

特别注意事项

1、微生物菌种应保藏于低温、清洁和干燥的地方,室温放置时问过长会导致菌种衰退;

2、菌种操作应在无菌条件下进行,防止杂菌污染:

3、斜面菌种保藏时间通常为1-2个月,应根据菌种状况及时转接;冻干菌种保藏时间通常为5~10年;

4、菌种使用过程中如出现杂菌污染或菌种生产性能下降，应及时与微生物菌种查询网联系或更换新的菌种。