迟缓优杆菌安瓿瓶冻干管打管说明书！

菌种简介

平台编号：bio-67697

规格：冻干粉

拉丁属名：Eggerthella lenta

菌株名称：迟缓优杆菌

注意事项：仅用于科学研究或者工业应用等非医疗目的，不可用于人类或动物的临床诊断或治疗，非药用，非食用

产品概述

Eggerthella lenta (Eggerth) Wade et al. 拉丁名

(ATCC? 43055?) 统一编号

Strain Designations 菌种别名 : MSMC 77-67 [CIP 104211] /

Type Strain 模式菌株: no /

Biosafety Level 生物安全等级: 1

Deposited As Eubacterium lentum (Eggerth) Prevot

Strain Designations 菌种别名 MSMC 77-67 [CIP 104211]

Application 用途 Quality control strain Susceptibility testing Isolation Abdominal wound, Wisconsin

Biosafety Level 生物安全等级 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format 提供形式 freeze-dried

Storage Conditions 保藏条件

Frozen（冷冻物）: -80 or colder

Freeze-Dried（冻干物）: 2 to 8

Live Culture: See Propagation Section

Preceptrol? no

Type Strain 模式菌株 no

Medium 培养基 ATCC? Medium 1761: Modified chopped meat medium (ATCC medium 1490) plus 0.5% arginine

Growth Conditions 生长条件

Temperature 培养温度: 37

Atmosphere 需氧情况: Anaerobic gas mixture, 80% N2-10%CO2-10%H2

Name of Depositor 寄存人 RJ Zabransky

Isolation 分离基物 Abdominal wound, Wisconsin

References 参考文献 Barry AL, Zabransky RJ. Eubacterium lentum ATCC 43055, a new reference strain for quality control of anaerobic susceptibility tests. J. Clin. Microbiol. 28: 2375-2376, 1990. PubMed: 2229368

Methods for Antimicrobial Susceptibility Testing of Anaerobic Bacteria. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M11-A7.

Wade WG, et al. The family Coriobacteriaceae: reclassification of Eubacterium exiguum (Poco et al. 1996) and Peptostreptococcus heliotrinreducens (Lanigan 1976) as Slackia exigua gen. nov., comb. nov. and Slackia heliotrinireducens gen. nov., comb. nov., and Eubacteriumlentum (Prevot 1938) as Eggerthella lenta gen. nov., comb. nov.. Int. J. Syst. Bacteriol. 49: 595-600, 1999. PubMed: 10319481

Sensititre? Anaerobe MIC Susceptibility. Thermo Scientific? Sensititre? Systems, Trek Diagnostic Systems.

Cross References Nucleotide (GenBank) : AX109565 Sequence 298 from Patent WO0123604.

安瓿瓶冻干管打管说明

一、打管说明

1、安瓿瓶开封：用浸过75％酒精的脱脂棉擦净安瓿管，用火焰加热其顶端，滴少量无菌水至加热顶端使之破裂，用锉刀或者镊子敲下已破裂的安瓿管顶端。

2、菌株恢复培养：用无菌吸管吸取0.3--0.5ml适宜的液体培养基，滴入安瓿管内，轻轻振荡，使冻干菌体溶解呈悬浮状。吸取全部菌体悬浮液，移植于1-2支建议的培养基试管中，并在建议的条件下培养。

3、注意事项：菌种活化前，请将安瓿管保存在6-10的环境下，某些菌种经过冷冻干燥保存后，延迟期较长，需要连续两次继代培养才能正常生长。

4、复苏后的菌种在传1-2代后使用。

5、暂不启开的安瓿及复苏后需保藏的斜面应于4中保藏。

二、特别注意事项

1、微生物菌种应保藏于低温、清洁和干燥的地方,室温放置时问过长会导致菌种衰退;

2、菌种操作应在无菌条件下进行,防止杂菌污染:

3、斜面菌种保藏时间通常为1-2个月,应根据菌种状况及时转接;冻干菌种保藏时间通常为5~10年;

4、菌种使用过程中如出现杂菌污染或菌种生产性能下降，应及时与微生物菌种查询网联系或更换新的菌种。