ATCC 39073 热醋穆尔氏菌 百欧博伟生物

菌种简介

平台编号：bio-74117

规格：freeze-dried

拉丁属名：Moorella thermoacetica

菌株名称：热醋穆尔氏菌

注意事项：仅用于科学研究或者工业应用等非医疗目的，不可用于人类或动物的临床诊断或治疗，非药用，非食用

产品概述

Moorella thermoacetica (Fontaine et al.) Collins et al. 菌株拉丁名

(ATCC? 39073?) 菌株编号

Type Strain 模式菌株 : no /

Biosafety Level 生物安全等级 : 1

Deposited As Clostridium thermoaceticum Fontaine et al.

Application 用途  Produces ATP synthase

Produces acetate Production of acetate from sugars Precipitation of cadmium

Biosafety Level 生物安全等级 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format 提供形式 freeze-dried

Storage Conditions 生长条件

Frozen 冷冻物 : -80 or colder

Freeze-Dried 冻干物 : 2 to 8

Live Culture 活菌 : See Propagation Section

Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims.

Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.

Preceptrol? no

Genome Sequenced Strain Moorella thermoacetica ATCC 39073, complete genome.

Type Strain 模式菌株 no

Comments 注释 Genome sequenced strain

Medium 模式菌株 ATCC? Medium 1203: CM4 medium

Growth Conditions 生长条件

Temperature 培养温度 : 55

Atmosphere 需氧情况 : Anaerobic gas mixture 80% N2-10% CO2-10% H2

Name of Depositor 寄存人 LG Ljungdahl

Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims.

Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished.

Chain of Custody 来源国家 ATCC <-- LG Ljungdahl <-- HG Wood

References 参考文献 Andreesen JR, et al. Fermentation of glucose, fructose, and xylose by Clostridium thermoaceticum:

Effect of metals on growth yield, enzymes, and the synthesis of acetate from CO2. J. Bacteriol. 114: 743-751, 1973. PubMed: 4706193

Cunningham DP, Lundie LL Jr.. Precipitation of cadmium by Clostridium thermoaceticum. Appl. Environ. Microbiol. 59: 7-14, 1993. PubMed: 8439169

安瓿瓶冻干管打管说明

一、打管说明

1、安瓿瓶开封：用浸过75％酒精的脱脂棉擦净安瓿管，用火焰加热其顶端，滴少量无菌水至加热顶端使之破裂，用锉刀或者镊子敲下已破裂的安瓿管顶端。

2、菌株恢复培养：用无菌吸管吸取0.3--0.5ml适宜的液体培养基，滴入安瓿管内，轻轻振荡，使冻干菌体溶解呈悬浮状。吸取全部菌体悬浮液，移植于1-2支建议的培养基试管中，并在建议的条件下培养。

3、注意事项：菌种活化前，请将安瓿管保存在6-10的环境下，某些菌种经过冷冻干燥保存后，延迟期较长，需要连续两次继代培养才能正常生长。

4、复苏后的菌种在传1-2代后使用。

5、暂不启开的安瓿及复苏后需保藏的斜面应于4中保藏。

二、特别注意事项

1、微生物菌种应保藏于低温、清洁和干燥的地方,室温放置时问过长会导致菌种衰退;

2、菌种操作应在无菌条件下进行,防止杂菌污染:

3、斜面菌种保藏时间通常为1-2个月,应根据菌种状况及时转接;冻干菌种保藏时间通常为5~10年;

4、菌种使用过程中如出现杂菌污染或菌种生产性能下降，应及时与微生物菌种查询网联系或更换新的菌种。