百欧博伟生物ATCC13883肺炎克雷伯菌肺炎亚种
(2022-05-03 10:21:22)
标签:
技术知识方法 |
分类: ATCC菌种 |
肺炎克雷伯菌肺炎亚种是Klebsiella属的微生物,原产地为中国。革兰氏阳性球菌,球状,直径0.5-1.5μm。单个、成对、四联,无动力,无芽孢。兼性厌氧,通常触酶阳性。主要用途为研究;教学;其他。
菌株简介
平台编号:bio-74064
规格:freeze-dried
拉丁属名:Klebsiella pneumoniae subsp. pneumoniae
中文名:肺炎克雷伯菌肺炎亚种
属:Klebsiella
原产地:中国
模式菌株:非模式菌株
注意事项:仅用于科学研究或者工业应用等非医疗目的,不可用于人类或动物的临床诊断或治疗,非药用,非食用
产品概述
Klebsiella pneumoniae subsp. pneumoniae (Schroeter) Trevisan 拉丁名
(ATCC® 13883™) 统一编号
Strain Designations 菌种别名 NCTC 9633 [NCDC 298-53, NCDC 410-68]
Application 用途 Assay of antimicrobial preservatives; Bacterial resistance testing adhesives; Bioresistance testing; Media testing; Quality control strain; Quality control strain for Abbott, API and bioMerieux Vitek, IDS, and Sensititre products; This strain is recommended by ATCC for use in the tests described in ASTM Standard Test Methods D4783-89 and E979-91 where only the taxon is specified.; Food testing; Respiratory research;
Biosafety Level 生物安全等级 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Antigenic Properties Type 3
Product Format 提供形式 freeze-dried
Storage Conditions 生长条件
Frozen 冷冻物: -80 or colder
Freeze-Dried 冻干物: 2 to 8
Live Culture 活物: See Propagation Section
Preceptrol® yes
Type Strain 模式菌株 yes
Antigenic Properties Type 3
Comments 注释 Also available as a component of ATCC GNI+ Organism set, 20-1001K.
Medium 培养基 ATCC® Medium 3: Nutrient agar or nutrient broth
Growth Conditions 生长条件
Temperature 培养温度: 37
Atmosphere 需氧情况 : Aerobic
Name of Depositor 寄存人 NCTC
Chain of Custody 来源国家 ATCC <-- NCTC <-- NCDC 298-53
References 参考文献 Skerman VB, et al. Approved lists of bacterial names. Int J Syst Bacteriol 30: 225-420, 1980.
Listeria species -- Biochemical Identification Method (Vitek GPI and GNI+). Gaithersburg, MD:AOAC International;AOAC "Official Methods of Analysis of the AOAC International" 992.19.
Standard Practice for Evaluating Water-Miscible Metalworking Fluid Bioresistance and Antimicrobial Pesticide. West Conshohocken, PA:ASTM International;ASTM Standard Test Method E 2275-03E01.
RapID™ SS/u QC Set. Remel™.
安瓿瓶冻干管打管说明
一、打管说明
1、安瓿瓶开封:用浸过75%酒精的脱脂棉擦净安瓿管,用火焰加热其顶端,滴少量无菌水至加热顶端使之破裂,用锉刀或者镊子敲下已破裂的安瓿管顶端。
2、菌株恢复培养:用无菌吸管吸取0.3--0.5ml适宜的液体培养基,滴入安瓿管内,轻轻振荡,使冻干菌体溶解呈悬浮状。吸取全部菌体悬浮液,移植于1-2支建议的培养基试管中,并在建议的条件下培养。
3、注意事项:菌种活化前,请将安瓿管保存在6-10的环境下,某些菌种经过冷冻干燥保存后,延迟期较长,需要连续两次继代培养才能正常生长。
4、复苏后的菌种在传1-2代后使用。
5、暂不启开的安瓿及复苏后需保藏的斜面应于4中保藏。
二、特别注意事项
1、微生物菌种应保藏于低温、清洁和干燥的地方,室温放置时问过长会导致菌种衰退;
2、菌种操作应在无菌条件下进行,防止杂菌污染:
3、斜面菌种保藏时间通常为1-2个月,应根据菌种状况及时转接;冻干菌种保藏时间通常为5~10年;
4、菌种使用过程中如出现杂菌污染或菌种生产性能下降,应及时与微生物菌种查询网联系或更换新的菌种。

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