ATCC 15591 石蜡节杆菌的微生物培养方法！

菌种简介

平台编号：Bio-83332

规格：Freeze-Dried

拉丁属名：Arthrobacter Paraffineus

菌株名称：石蜡节杆菌

用途：ATCC原装进口

注意事项：仅用于科学研究或者工业应用等非医疗目的不可用于人类或动物的临床诊断或治疗，非药用，非食用（产品信息以出库为准）

详细信息

Arthrobacter paraffineus 拉丁名

(ATCC? 15591?) 统一编号

Strain Designations 别名 2411

Application 用途 Degrades hydrocarbons； Produces citric acid citrate ；Produces glutamic acid L-glutamate, L-glutamic acid, glutamate ；Production of citric acid by fermentation of hydrocarbons ；Production of L-glutamic acid utilizing hydrocarbons in the presence of a dispersing agent and antibiotics

Isolation 分离基物 Soil

Biosafety Level 生物安全等级 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format 提供形式 freeze-dried

Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.

Preceptrol? no

Type Strain 模式菌种 no

Medium 培养基 ATCC? Medium 3: Nutrient agar or nutrient broth

Growth Conditions 生长条件

Temperature 培养温度: 30.0°C

Name of Depositor 寄存者 Kyowa Ferm. Ind. Co., Ltd.

U.S. Patent Number 3,691,012

Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.

Chain of Custody 来源国家 ATCC <<--Kyowa Ferm. Ind. Co., Ltd.<<--K. Tanaka and K. Kimura

Isolation 分离基物 Soil

References 参考文献 Tanaka K, et al. Process for producing citric acid. US Patent 3,691,012 dated Sep 12 1972 Kimura K, Nakanishi T. Production of citric acid, isocitric acid, and microbial cells by fermentation. US Patent 3,873,424 dated Mar 25 1975 Tanaka K, et al. Process for producing L-glutamic acid.

US Patent 3,764,473 dated Oct 9 1973 Tanaka K, Kimura K. Process for preparing amino acids from hydrocarbons. US Patent 3,655,510 dated Apr 11 1972

微生物培养方法

1、平板划线分离法：把混杂在一起的微生物或同一微生物群体中的不同细胞用接种环圈在平板培养基表面,通过分区划线稀释而得到较多独立分布的单个细胞,经培养后生长繁殖成单菌落。

2、稀释涂布平板法：将菌液进行一系列的梯度稀释,然后将不同稀释度的菌液分别涂布到琼脂固体培养基的表面进行培养,在稀释度足够高的菌液里,聚集在一起的微生物将被分散成单个细胞,从而能在培养基表面形成单个菌落。

上述两种方法虽然都可以实现观察菌落特征的目的,但平板划线分离法不能对菌落计数,而稀释涂布平板法培养过程复杂,对平板的要求比较高,可参照以下步骤:

a、制备平板培养基将氯化钠蔗糖琼脂培养基加热溶化并冷却至65,向氯化钠蔗糖琼脂培养基中加入克林霉素溶液3~5ml并混合均匀,然后取20ml混合均匀的培养基溶液倒入培养皿,轻轻摇动培养皿,使培养基溶液均匀分布在培养皿底部,待培养基溶液凝固后即得平板培养基。

b、制备活性污泥混合液称取活性污泥土样15g,放入盛100ml无菌水并带有玻璃珠的三角烧瓶中,振摇15~20min混合均匀,用一支1ml无菌吸管从中吸取1ml土壤悬液加入盛有9ml无菌水的大试管中充分混匀,制成活性污泥混合液。

c、培养基涂布从活性污泥混合液中吸取0.2ml,滴在平板培养基表面的中央位置,用无菌玻璃涂棒将活性污泥混合液沿同心圆方向轻轻地向外扩展,使之分布均匀。