数据分为2组：

*-1 为group1

*-2为group2

R代码

library(pheatmap)

data<-read.table("data.txt",header=T)

#主要就是annotation起作用

annotation = data.frame(Treat = factor(1:10%/%6,labels=c("group1","group2")))

rownames(annotation) = colnames(data)

Var1 = c("yellow", "blue")

ann_colors = list(Treat=Var1)

pheatmap(data, annotation = annotation, annotation_colors = ann_colors, main = "Example",border_color=NA,cluster_row=F,cluster_col=F,fontsize=10,cellwidth = 20, fontsize_row=10,cellheight = 10,colorRampPalette(c("green", "black", "red")) (255))