荧光共定位分析(colocalization)编辑词条发表评论(0)

在荧光显微镜 ，观察共存是指空间之间的重叠两个（或更多）不同的荧光标记，每一个独立的发射波长，看看不同的“目标”是位于细胞的同一地区或者非常接近另一个。 The definition can be split into two different phenomena, co-occurrence, which refers to the presence of two (possibly unrelated) fluorophores in the same pixel, and correlation, a much more significant statistical relationship between the fluorophores indicative of a biological interaction. [ 1 ] .这个定义可以分为两个不同的现象，共生，这是指两个荧光基团的存在（可能无关）在相同的像素，和相关性，表明了一个更为重要的生物荧光分子相互作用的统计之间的关系。 [ 1] 。 This technique is important to many cell biological and physiological studies during the demonstration of a relationship between pairs of bio-molecules.这种技术是很重要的，在许多细胞之间的一个生物分子对生物和生理关系的实证研究。

能够表现出分子的相关性，一对生物大大提高了埃里克介绍曼德斯谁是阿姆斯特丹大学的皮尔逊相关系数为显微镜[2] ，以及重叠系数等系数，其中的“”货币供应量M1和M2有被证明是最流行的和有用的[3] [4] 。 The purpose of using coefficients is to characterize the degree of overlap between images, usually two channels in a multidimensional microscopy image recorded at different emission wave lengths.利用系数的目的是描述图像之间的重叠度，在一个多层面的显微镜在不同发射波长录制的图像通常有两个渠道。 A popular approach was introduced by Sylvain Costes, who utilized Pearson's Correlation Coefficient as a tool for setting the thresholds required by M1 and M2 in an objective fashion [ 5 ] .一种流行的做法是时尚介绍维尔托德科斯特斯，谁在1平方米的目标，并利用皮尔逊的相关性的货币供应量M1为系数作为一种工具设置的门槛要求[5] 。 Costes approach makes the assumption that only positive correlations are of interest, and does not provide a useful measurement of PCC.科斯特斯方法使得假设，认为只有正相关的利益，并且不提供一个有用的政协测量。 


Although the use of coefficients can significantly improve the reliability of colocalization detection, it depends on the number of factors, including the conditions of how samples with fluorescence were prepared and how images with colocalization were acquired and processed.虽然系数使用可显着提高检测的可靠性共存，这取决于如何与样品制备荧光图像，以及如何与被收购和加工共存于许多因素，其中包括条件。 Studies should be conducted with great caution, and after careful background reading.应该进行研究，非常谨慎，并经过背景阅读。 Currently the field is dogged by confusion and a standarized approach is yet to be firmly established [ 6 ] .目前，该场是一个顽强的混乱和standarized方式尚未确立的[6] 。 Attempts to rectify this include re-examination and revision of some of the coefficients [ 7 ] and application of a factor to correct for noise [ 8 ] .试图纠正这包括重新审查和调整系数的一些[7]和要素的应用，以正确的噪声为[8] 。 Some authors have both proposed propocols [ 9 ] and sell accompanying software .有些作者都提出propocols [9]和销售随附软件 。 In addition, due to the tendency of fluorescence images to contain a certain amount of out-of-focus noise, they usually require pre-processing prior to quantification. [ 10 ] .此外，由于荧光图像倾向含有一定量的集中噪音外的，他们通常需要预先处理之前，量化。 [10] 。 This pre-processing can be done using background thresholding or deconvolution.这种预处理可以使用背景阈值或解卷积。 

Adler et al (2008). “复制的噪音纠正”关系的共定位的精确测量。 [1] "Replicate based noise corrected correlations for accurate measurements of colocalization". [1] 
2.^曼德斯等人（1992年）。 ^ Manders et al (1992). “动态三维模式复制在S期，共聚焦显微镜分析和双标记的DNA。” [2] "Dynamics of three-dimensional replication patterns during the S-phase, analysed by double labelling of DNA and confocal microscopy." [2]