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在免疫排斥肿瘤中跟踪T淋巴细胞--俞婧佳翻译

(2007-03-02 13:46:01)
 

Monitoring T-lymphocyte trafficking in tumors undergoing immune rejection.

在免疫排斥肿瘤中跟踪T淋巴细胞

Activated T cells, isolated from animals that had rejected a tumor (E.G7-OVA) expressing chicken ovalbumin, were labeled with citrated superparamagnetic iron oxide nanoparticles at an intracellular iron concentration of up to 0.5 pg/cell. 从对表达鸡卵白蛋白的肿瘤(E.G7-OVA)有排斥能力的动物中分离出活化的T细胞,再用超顺磁性的三氧化二铁纳米粒标记,使其细胞内的铁含量达到0.5pg/细胞。

Injection of these labeled T cells into animals bearing E.G7-OVA tumors undergoing immune rejection resulted in tumor infiltration of these cells, which was detectable as a heterogeneous decrease in intensity in T(2)-weighted MR images. 将这些标记的T细胞注射到经过免疫排斥的荷E.G7-OVA肿瘤的动物体内,发现这些细胞渗入肿瘤周围, T(2)加权的磁共振影像可见不均一的减少。

T-cell infiltration was confirmed by immunohistochemical staining of tumor sections obtained postmortem and was shown to colocalize with iron that had been stained using Prussian blue. 对尸体解剖后的切片进行的免疫组化染色证实,T细胞已经渗入肿瘤,同时普鲁士蓝染色也证实,它与铁离子在肿瘤组织内有共同的定位。

Tumor rejection was correlated with the uptake of labeled T cells, since the infiltration of labeled T cells was only observed in those tumors that went on to regress. 由于标记T细胞的渗入现象只能在那些退化的肿瘤中被发现,说明肿瘤免疫排斥现象与标记T细胞的吸收有关。

This technique should assist in the elucidation of those factors that are important in mediating tumor immune rejection. 此技术有助于阐明那些参与介导肿瘤免疫排斥的重要因素。

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