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标签:

股票

免疫学

流式

分类: 常见问题

无信号/荧光强度弱

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问题

 

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SAFE™ Cytokines and Growth Factors
SAFE™为eBioscience新研发的冻干重组蛋白,该重组蛋白无血清,不含动物性成分,内毒素含量低。

  • human Activin A
  • human Activin B
  • human BMP-2
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标签:

流式

样本处理

免疫学

杂谈

分类: 流式

 

方案A:组织培养细胞的制备。

 

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标签:

流式

红细胞裂解

杂谈

分类: 流式

材料针对外周血,组织中红细胞。

方法:

1.   裂解

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Platinum ELISA Kits

Performance above the gold standard.

> Platinum ELISA Products

Platinum ELISA kits offer over 150 quality-validated kits for human, mouse, rat, monkey, and pig target analysis. Stringent in-house development, manufacture and quality-validation processes ensure the highest possible ELISA performance.

A complete, validated ELISA solution right out of the box.

Advantages of Platinum ELISA Kits

  • Performance – designed for quality performance, right out of the box.
  • Validated – tested for accurate and relevant ELISA results from serum, plasma or cell culture supernatants.
  • Highly Reproducible – Stringent QC criteria ensure lot-to-lot consistency.
  • Excellent Sensitivity

Platinum ELISA Kits. Performance above the Gold Standard.

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Specificity

Clone

Format

Cat. No.

 

 

 

 

CD4

*YKIX302.9

FITC

11-5040

CD5

*YKIX322.3

PerCP-eFluor® 710

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00-5333-54  50 ml RMB 1500.00

00-5333-57 200 ml RMB 4800.00

 

 

eBioscience 1-step Fix/Lyse Solution (10X)
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Regulators of the Immune System

 

Regulatory T cells (Tregs) are critical to the maintenance of immune cell homeostasis as evidenced by the catastrophic consequences of genetic or physical ablation of the Treg population. Specifically, Treg cells maintain order in the immune system by enforcing a dominant negative regulation on other immune cells. Broadly classified into natural or adaptive (induced) Tregs; natural Tregs are CD4+CD25+ T-cells which develop, and emigrate from the thymus to perform their key role in immune homeostasis. Adaptive Tregs are non-regulatory CD4+ T-cells which acquire CD25 (IL-2Rα) expression_r outside of the thymus, and are typically induced by inflammation and disease processes, such as autoimmunity and cancer.

Precise understanding of the immunosuppressi

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(2010-04-16 13:24)
标签:

elispot

b

cells

杂谈

分类: ELISpot

In spite of the first described use of the ELISpot being that of detecting antibody-secreting B cells, there have so far not been any designated ELISpot kits for this application. To accommodate this need for validated reagents and protocols we have recently developed a series of kits for detection of human and mouse B cells.

As schematically illustrated below, the B-cell ELISpot may be used both for determining the number of antigen-specific B cells (A) as well as the total number of antibody-secreting cells (B). In the antigen-specific setting, the assay can be performed in either of two ways, one where the antigen is immobilized on the ELISpot plate and one where the same antigen is instead used for detection. While the first approach has been the one almost exclusively used, we have found that using antigen for detection may have several advantages including better sensitivity and a lesser need for antigen.

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